Hotspot

psbC-trnS-TGA

LSC · 34775-35013 · 239 bp

Back to Roldana

Pi 0.046
MarkerSeek score 71.90
Primer available yes
Label psbC-trnS-TGA

Feature Metadata

label_name
psbC-trnS-TGA
feature_type
igs
region
LSC
length_bp
239
variable_sites
11
pi
0.046
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-TGA_p1 TCTTCGTAGGGCATTTGTGG GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.9 58.0 434-439 bp 1.0 81.0
psbC-trnS-TGA_p2 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 351-356 bp 1.0 81.0
psbC-trnS-TGA_p3 TCTTCGTAGGGCATTTGTGG GTCTTTCGGAGAGATGGCTG 50.0 55.0 57.9 58.1 442-447 bp 1.0 80.6
psbC-trnS-TGA_p4 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 359-364 bp 1.0 80.6
psbC-trnS-TGA_p5 TCTTCGTAGGGCATTTGTGG TCTTTCGGAGAGATGGCTGA 50.0 50.0 57.9 58.1 441-446 bp 1.0 79.9