Hotspot

psbI-trnS-GCT

LSC · 8448-8587 · 140 bp

Back to Roldana

Pi 0.0441

MarkerSeek score 80.50

Primer available yes

Label psbI-trnS-GCT

Feature Metadata

label_name: psbI-trnS-GCT
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 6
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCT_p1	CGTAATCCAGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	240-256 bp	1.0	77.6
psbI-trnS-GCT_p2	CGTAATCCAGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	240-256 bp	1.0	75.2
psbI-trnS-GCT_p3	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	259-275 bp	1.0	74.3
psbI-trnS-GCT_p4	CGTAATCCAGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.1	58.6	231-247 bp	1.0	73.5
psbI-trnS-GCT_p5	CGTAATCCAGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	204 bp	0.5	49.4