Hotspot

trnQ-TTG-psbK

LSC · 7375-7730 · 356 bp

Back to Roldana

Pi 0.0225

MarkerSeek score 66.60

Primer available yes

Label trnQ-TTG-psbK

Feature Metadata

label_name: trnQ-TTG-psbK
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 8
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-TTG-psbK_p1	GTTGCCTTACCACTTGGCTA	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.8	57.8	458 bp	1.0	78.5
trnQ-TTG-psbK_p2	TTGCCTTACCACTTGGCTAC	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.8	57.8	457 bp	1.0	78.5
trnQ-TTG-psbK_p3	GTTGCCTTACCACTTGGCTA	CATAGGCTTCGGGCAATTTG	50.0	50.0	57.8	57.5	468 bp	1.0	75.8
trnQ-TTG-psbK_p4	TTGCCTTACCACTTGGCTAC	CATAGGCTTCGGGCAATTTG	50.0	50.0	57.8	57.5	467 bp	1.0	75.8
trnQ-TTG-psbK_p5	TTGCCTTACCACTTGGCTAC	ATAGGCTTCGGGCAATTTGG	50.0	50.0	57.8	58.6	466 bp	1.0	75.0