Hotspot

ycf3

LSC · 42822-44816 · 1995 bp

Pi 0.00201

MarkerSeek score 42.30

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1995
variable_sites: 7
pi: 0.00201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TCTCATGTCATGGTCATGGT	CCGAGGGGGTAATGAGAAGA	45.0	55.0	56.2	58.2	2124-2125 bp	1.0	64.4
ycf3_p2	TCTCATGTCATGGTCATGGT	GATAATTTTCCCCGAGCCGA	45.0	50.0	56.2	57.8	2140-2141 bp	1.0	64.1
ycf3_p3	TCTCATGTCATGGTCATGGT	ATAATTTTCCCCGAGCCGAG	45.0	50.0	56.2	57.7	2139-2140 bp	1.0	64.0
ycf3_p4	TTCTCATGTCATGGTCATGGT	CCGAGGGGGTAATGAGAAGA	42.9	55.0	57.0	58.2	2125-2126 bp	1.0	62.6
ycf3_p5	TGTCATGGTCATGGTAATGGT	CCGAGGGGGTAATGAGAAGA	42.9	55.0	57.0	58.2	2119-2120 bp	1.0	62.5