Hotspot

trnQ-UUG

LSC · 7128-7199 · 72 bp

Back to Rohdea

Pi 0.0139
MarkerSeek score 52.80
Primer available yes
Label trnQ-UUG

Feature Metadata

label_name
trnQ-UUG
feature_type
tRNA
region
LSC
length_bp
72
variable_sites
2
pi
0.0139
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG_p1 AACAAAGAAGGGGTTGGGTC TATTTGGGCTGGGATTGACG 50.0 50.0 57.9 57.9 246 bp 1.0 79.6
trnQ-UUG_p2 GAACAAAGAAGGGGTTGGGT TATTTGGGCTGGGATTGACG 50.0 50.0 57.9 57.9 247 bp 1.0 79.6
trnQ-UUG_p3 AGAACAAAGAAGGGGTTGGG TATTTGGGCTGGGATTGACG 50.0 50.0 57.6 57.9 248 bp 1.0 77.2
trnQ-UUG_p4 AACAAAGAAGGGGTTGGGTC CTGGGATTGACGAATGACCA 50.0 50.0 57.9 57.6 238 bp 1.0 76.8
trnQ-UUG_p5 GAACAAAGAAGGGGTTGGGT CTGGGATTGACGAATGACCA 50.0 50.0 57.9 57.6 239 bp 1.0 76.8