Hotspot

ccsA-ndhD

SSC · 117333-117563 · 231 bp

Back to Rohdea

Pi 0.0281
MarkerSeek score 70.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
231
variable_sites
10
pi
0.0281
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGAATTCGTCACTTGTAGCG TCGCTATCAGTTGACAAGGT 50.0 45.0 57.3 56.6 404 bp 1.0 65.2
ccsA-ndhD_p2 CGAATTCGTCACTTGTAGCG TTCGCTATCAGTTGACAAGGT 50.0 42.9 57.3 57.3 405 bp 1.0 63.1
ccsA-ndhD_p3 ATTCGTCACTTGTAGCGTCT TCGCTATCAGTTGACAAGGT 45.0 45.0 57.0 56.6 401 bp 1.0 62.5
ccsA-ndhD_p4 CGAATTCGTCACTTGTAGCG TCAGTTGACAAGGTACAAGCT 50.0 42.9 57.3 57.2 398 bp 1.0 61.9
ccsA-ndhD_p5 CGAATTCGTCACTTGTAGCG TTTCGCTATCAGTTGACAAGGT 50.0 40.9 57.3 58.0 406 bp 1.0 60.5