Hotspot

ndhF-rpl32

SSC · 114082-115096 · 1015 bp

Back to Rohdea

Pi 0.00411
MarkerSeek score 61.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1015
variable_sites
8
pi
0.00411
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACTGGAAATGGAAGAAAGGGT ACTGACTTTGCTAAAGAAAAAGCT 42.9 33.3 57.1 57.5 1181-1205 bp 1.0 40.7
ndhF-rpl32_p2 AACTGGAAATGGAAGAAAGGGT ACTGACTTTGCTAAAGAAAAAGCT 40.9 33.3 57.8 57.5 1182-1206 bp 1.0 40.7
ndhF-rpl32_p3 ACTGGAAATGGAAGAAAGGGT ACTGACTTTGCTAAAGAAAAAGC 42.9 34.8 57.1 56.2 1181-1205 bp 1.0 40.7
ndhF-rpl32_p4 TGTTGCTGTCGGAATAAGAAGA ACTGACTTTGCTAAAGAAAAAGCT 40.9 33.3 57.7 57.5 1227-1251 bp 1.0 40.7
ndhF-rpl32_p5 ACTGGAAATGGAAGAAAGGGT CTGACTTTGCTAAAGAAAAAGCT 42.9 34.8 57.1 55.9 1180-1204 bp 1.0 40.7