Hotspot

rps12-trnV-GAC

IRb · 100294-102134 · 1841 bp

Back to Rohdea

Pi 0.0206
MarkerSeek score 62.30
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1841
variable_sites
57
pi
0.0206
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA CGGGGGAGCAAGTCAAAATA 45.0 50.0 57.7 57.9 1998-2029 bp 1.0 79.6
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1937-1968 bp 1.0 79.3
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1941-1972 bp 1.0 79.3
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1936-1967 bp 1.0 79.3
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1939-1970 bp 1.0 78.3