Hotspot

trnF-GAA-ndhJ

LSC · 48737-49456 · 720 bp

Pi 0.00162

MarkerSeek score 51.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 2
pi: 0.00162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATGCCAGAAAGTTGGATGGG	50.0	50.0	58.1	58.1	829-835 bp	1.0	79.2
trnF-GAA-ndhJ_p2	CTAGTATGATGCGCGGGAAA	ATGCCAGAAAGTTGGATGGG	50.0	50.0	57.9	58.1	890-896 bp	1.0	78.4
trnF-GAA-ndhJ_p3	GGAAATGGTCGGGATAGCTC	ATGCCAGAAAGTTGGATGGG	55.0	50.0	57.8	58.1	875-881 bp	1.0	78.2
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	ATGCCAGAAAGTTGGATGGG	50.0	50.0	58.2	58.1	872-878 bp	1.0	77.9
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATGCCAGAAAGTTGGATGGG	50.0	50.0	58.2	58.1	871-877 bp	1.0	77.9