Hotspot

rpoB-trnC-GCA

LSC · 27296-28488 · 1193 bp

Back to Rohdea

Pi 0.00265
MarkerSeek score 59.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1193
variable_sites
5
pi
0.00265
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGCCAAGCCCTGATTAATGA TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.3 57.5 1331-1356 bp 1.0 73.7
rpoB-trnC-GCA_p2 CGCCAAGCCCTGATTAATGA CCTTACCACTTGGCCATGTC 50.0 55.0 58.3 58.5 1310-1335 bp 1.0 73.6
rpoB-trnC-GCA_p3 TCCCTCATTTCCATTCCGGA TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.4 57.5 1259-1284 bp 1.0 73.0
rpoB-trnC-GCA_p4 TCCCTCATTTCCATTCCGGA CCTTACCACTTGGCCATGTC 50.0 55.0 58.4 58.5 1238-1263 bp 1.0 72.9
rpoB-trnC-GCA_p5 TTCCCTCATTTCCATTCCGG TAAAGGATTTGCAGTCCCCC 50.0 50.0 57.6 57.5 1260-1285 bp 1.0 72.8