Hotspot

trnR-UCU-atpA

LSC · 10761-10878 · 118 bp

Back to Rohdea

Pi 0.0127
MarkerSeek score 60.30
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
118
variable_sites
2
pi
0.0127
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGAATGAAAAGCGTCCATTGT ACTGAGGAAGCAGAAACCCT 42.9 50.0 57.4 58.6 278 bp 1.0 63.5
trnR-UCU-atpA_p2 GGAATGAAAAGCGTCCATTGT CTGAGGAAGCAGAAACCCTT 42.9 50.0 57.4 57.4 277 bp 1.0 63.4
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC ACTGAGGAAGCAGAAACCCT 47.6 50.0 57.3 58.6 218 bp 1.0 62.7
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC CTGAGGAAGCAGAAACCCTT 47.6 50.0 57.3 57.4 217 bp 1.0 62.6
trnR-UCU-atpA_p5 AGGAATGAAAAGCGTCCATTG ACTGAGGAAGCAGAAACCCT 42.9 50.0 57.1 58.6 279 bp 1.0 61.2