Hotspot

trnS-GCU-trnG-UCC

LSC · 8445-9775 · 1331 bp

Back to Rohdea

Pi 0.00482
MarkerSeek score 65.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1331
variable_sites
12
pi
0.00482
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1448-1490 bp 1.0 72.6
trnS-GCU-trnG-UCC_p2 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 1452-1494 bp 1.0 72.6
trnS-GCU-trnG-UCC_p3 ATCAGAGGGAACGGAGAGAG ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 1447-1489 bp 1.0 72.6
trnS-GCU-trnG-UCC_p4 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCAC 55.0 45.0 57.9 56.8 1448-1490 bp 1.0 72.6
trnS-GCU-trnG-UCC_p5 GATCAGAGGGAACGGAGAGA CGAACGAATCGCACTTTTACC 55.0 47.6 57.9 58.2 1451-1493 bp 1.0 72.5