Hotspot

ycf1

IRb · 112209-113349 · 1141 bp

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Pi 0

MarkerSeek score 27.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1141
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	ACTTTGTTCGTTGGAGCCAT	55.0	45.0	58.0	58.0	2091-2099 bp	1.0	79.9
ycf1_p2	CCCGGAACTTCTTCGTAGTG	ACTTTGTTCGTTGGAGCCAT	55.0	45.0	58.0	58.0	1996-2004 bp	1.0	79.9
ycf1_p3	CCGGAACTTCTTCGTAGTGG	ACTTTGTTCGTTGGAGCCAT	55.0	45.0	58.0	58.0	1995-2003 bp	1.0	79.9
ycf1_p4	AGGAACAACGGGGGATTAGA	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.0	58.0	2082-2090 bp	1.0	79.6
ycf1_p5	TCCCGGAACTTCTTCGTAGT	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.1	58.0	1997-2005 bp	1.0	79.3