Hotspot

rps18

LSC · 71728-72039 · 312 bp

Back to Rodgersia

Pi 0

MarkerSeek score 23.30

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 312
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGGATTTTCAGGGATAAGGA	TCTGCGTTTGAGTTCGGATT	40.0	45.0	52.9	57.8	391 bp	1.0	40.0
rps18_p2	AGGATTTTCAGGGATAAGGAA	TCTGCGTTTGAGTTCGGATT	38.1	45.0	53.9	57.8	391 bp	1.0	40.0
rps18_p3	AGGATTTTCAGGGATAAGGA	CTGCGTTTGAGTTCGGATTG	40.0	50.0	52.9	57.8	390 bp	1.0	40.0
rps18_p4	AGGATTTTCAGGGATAAGGAA	CTGCGTTTGAGTTCGGATTG	38.1	50.0	53.9	57.8	390 bp	1.0	40.0
rps18_p5	AGGATTTTCAGGGATAAGGA	ATCTGCGTTTGAGTTCGGAT	40.0	45.0	52.9	57.6	392 bp	1.0	40.0