Hotspot

ndhF

SSC · 113392-115638 · 2247 bp

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Pi 0.00089

MarkerSeek score 43.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 2
pi: 0.00089
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAACCTCTTGTGACCCGTC	TAATGGGTCAAGCTCGAAGA	50.0	45.0	58.0	56.3	2987-2995 bp	1.0	66.0
ndhF_p2	CTTGTGACCCGTCTTTTCGA	TAATGGGTCAAGCTCGAAGA	50.0	45.0	58.1	56.3	2980-2988 bp	1.0	65.2
ndhF_p3	TCTTGTGACCCGTCTTTTCG	TAATGGGTCAAGCTCGAAGA	50.0	45.0	58.1	56.3	2981-2989 bp	1.0	65.2
ndhF_p4	CCTCTTGTGACCCGTCTTTT	TAATGGGTCAAGCTCGAAGA	50.0	45.0	57.7	56.3	2983-2991 bp	1.0	64.2
ndhF_p5	AAAACCTCTTGTGACCCGTC	TTAATGGGTCAAGCTCGAAGA	50.0	42.9	58.0	57.0	2988-2996 bp	1.0	64.1