Hotspot

clpP

LSC · 73730-75729 · 2000 bp

Back to Rodgersia

Pi 0.001

MarkerSeek score 46.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2000
variable_sites: 2
pi: 0.001
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AAAATCACAGATTTGCGCGA	ACGTTTCCACATCAAAGTGA	40.0	40.0	57.0	55.5	2086-2087 bp	1.0	52.4
clpP_p2	AAATCACAGATTTGCGCGAA	ACGTTTCCACATCAAAGTGA	40.0	40.0	57.0	55.5	2085-2086 bp	1.0	52.4
clpP_p3	AATCACAGATTTGCGCGAAA	ACGTTTCCACATCAAAGTGA	40.0	40.0	57.0	55.5	2084-2085 bp	1.0	52.4
clpP_p4	AAATCACAGATTTGCGCGAA	ACGTTTCCACATCAAAGTGAA	40.0	38.1	57.0	56.3	2085-2086 bp	1.0	50.7
clpP_p5	AATCACAGATTTGCGCGAAA	ACGTTTCCACATCAAAGTGAA	40.0	38.1	57.0	56.3	2084-2085 bp	1.0	50.7