Hotspot

ndhF-rpl32

SSC · 115639-116774 · 1136 bp

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Pi 0.00264

MarkerSeek score 60.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1136
variable_sites: 3
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACGAAAGGT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1276 bp	1.0	77.9
ndhF-rpl32_p2	AAAAAGGAGAAGCCCCACTC	TTAACGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1311 bp	1.0	77.6
ndhF-rpl32_p3	GAAAAAGGAGAAGCCCCACT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1312 bp	1.0	77.6
ndhF-rpl32_p4	CTGGAAGTGGAACGAAAGGT	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.7	57.9	1284 bp	1.0	76.9
ndhF-rpl32_p5	AAGATTTTTGGTTGCCGTCG	TTAACGCTGCCCAATATCCC	45.0	50.0	57.6	58.0	1332 bp	1.0	76.7