Hotspot

psbZ-trnG-UCC

LSC · 38323-39043 · 721 bp

Back to Rodgersia

Pi 0.0042

MarkerSeek score 62.10

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 3
pi: 0.0042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	872-879 bp	1.0	76.3
psbZ-trnG-UCC_p2	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	873-880 bp	1.0	75.0
psbZ-trnG-UCC_p3	GTATTTGCTTCTCCTGACGGT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	875-882 bp	1.0	71.3
psbZ-trnG-UCC_p4	TGTATTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	876-883 bp	1.0	71.3
psbZ-trnG-UCC_p5	CCCGTTGTATTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	881-888 bp	1.0	69.9