Hotspot

trnE-UUC-trntT

LSC · 32706-33572 · 867 bp

Back to Rodgersia

Pi 0

MarkerSeek score 59.90

Primer available yes

Label trnE-UUC-trntT

Feature Metadata

label_name: trnE-UUC-trntT
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trntT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	998-1008 bp	1.0	79.5
trnE-UUC-trntT_p2	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	963-973 bp	1.0	78.7
trnE-UUC-trntT_p3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	960-970 bp	1.0	78.7
trnE-UUC-trntT_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	939-949 bp	1.0	78.5
trnE-UUC-trntT_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	995-1005 bp	1.0	78.3