Hotspot

trnS-trnG-GCC

LSC · 9057-9841 · 785 bp

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Pi 0.00127
MarkerSeek score 59.50
Primer available yes
Label trnS-trnG-GCC

Feature Metadata

label_name
trnS-trnG-GCC
feature_type
igs
region
LSC
length_bp
785
variable_sites
1
pi
0.00127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 854 bp 1.0 51.8
trnS-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 853 bp 1.0 51.8
trnS-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 857 bp 1.0 50.7
trnS-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 856 bp 1.0 50.7
trnS-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 855 bp 1.0 50.7