Hotspot

ycf1

SSC · 117611-121456 · 3846 bp

Back to Rochelia

Pi 0.00373

MarkerSeek score 43.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3846
variable_sites: 37
pi: 0.00373
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAATGGTATCCCAGGCTTC	CCTTTTTCTTTTGATATCTTCGAACT	55.0	30.8	57.7	56.5	137-170 bp	1.0	47.4
ycf1_p2	GGAATGGTATCCCAGGCTTC	CCTTTTTCTTTTGATATCTTCGAAC	55.0	32.0	57.7	55.3	137-170 bp	1.0	47.4
ycf1_p3	TCCTTGTGCAAAGGGAATGG	CCTTTTTCTTTTGATATCTTCGAACT	50.0	30.8	58.4	56.5	150-183 bp	1.0	47.2
ycf1_p4	TCCTTGTGCAAAGGGAATGG	CCTTTTTCTTTTGATATCTTCGAAC	50.0	32.0	58.4	55.3	150-183 bp	1.0	47.2
ycf1_p5	CCTTGTGCAAAGGGAATGGT	CCTTTTTCTTTTGATATCTTCGAACT	50.0	30.8	58.7	56.5	149-182 bp	1.0	47.2