Hotspot

ndhA

SSC · 113670-115760 · 2091 bp

Back to Rochelia

Pi 0.00249

MarkerSeek score 54.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2091
variable_sites: 15
pi: 0.00249
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTCTTGTTTGAGAGAAGTTGTGA	CCGCCGGGTTTTATCAATTT	37.5	45.0	58.0	57.0	2232-2249 bp	1.0	41.7
ndhA_p2	TCTCTTGTTTGAGAGAAGTTGTGA	GCCGGGTTTTATCAATTTGCA	37.5	42.9	58.0	58.0	2230-2247 bp	1.0	41.5
ndhA_p3	CTCTTGTTTGAGAGAAGTTGTGA	CCGCCGGGTTTTATCAATTT	39.1	45.0	56.7	57.0	2231-2248 bp	1.0	41.5
ndhA_p4	TCTCTTGTTTGAGAGAAGTTGTG	CCGCCGGGTTTTATCAATTT	39.1	45.0	56.7	57.0	2232-2249 bp	1.0	41.5
ndhA_p5	CTCTTGTTTGAGAGAAGTTGTGA	GCCGGGTTTTATCAATTTGCA	39.1	42.9	56.7	58.0	2229-2246 bp	1.0	41.5