Hotspot

rpl32-trnL-UAG

SSC · 107715-108290 · 576 bp

Back to Rochelia

Pi 0.00752

MarkerSeek score 39.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 576
variable_sites: 13
pi: 0.00752
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGGAGGGGGTATTGGACA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	711-722 bp	1.0	80.6
rpl32-trnL-UAG_p2	AAAGGAGGGGGTATTGGACA	CTTAGTGGAAAATGCCCCGA	50.0	50.0	57.9	57.9	1060-1066 bp	1.0	80.4
rpl32-trnL-UAG_p3	AAAGGAGGGGGTATTGGACA	ACTGAAGAAAAACGGCGGAT	50.0	45.0	57.9	58.1	1406-1412 bp	1.0	80.2
rpl32-trnL-UAG_p4	AAAGTTTTGTTGTGCGGCAA	ACTGAAGAAAAACGGCGGAT	40.0	45.0	57.9	58.1	1337-1343 bp	0.833333	72.3
rpl32-trnL-UAG_p5	AAGTTTTGTTGTGCGGCAAA	ACTGAAGAAAAACGGCGGAT	40.0	45.0	57.9	58.1	1336-1342 bp	0.833333	72.3