Hotspot

psbB-psbT

LSC · 69656-69827 · 172 bp

Back to Rochelia

Pi 0.00388

MarkerSeek score 29.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 2
pi: 0.00388
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TTCTTCGGACACATTTGGCA	AATAGAGCCATTGCAACCCC	45.0	50.0	58.0	58.2	869-879 bp	1.0	79.3
psbB-psbT_p2	GCAACTACTCCCGTCGAATT	AATAGAGCCATTGCAACCCC	50.0	50.0	58.0	58.2	1475-1485 bp	1.0	79.0
psbB-psbT_p3	GGGCGTGAACTTTTTGTACG	AATAGAGCCATTGCAACCCC	50.0	50.0	58.0	58.2	1205-1215 bp	1.0	79.0
psbB-psbT_p4	AGCTGGCTCAATGGATAACG	AATAGAGCCATTGCAACCCC	50.0	50.0	58.0	58.2	1278-1288 bp	1.0	78.8
psbB-psbT_p5	ACAAAGAAGGGCGTGAACTT	AATAGAGCCATTGCAACCCC	45.0	50.0	57.9	58.2	1213-1223 bp	1.0	78.7