Hotspot

petA-psbJ

LSC · 59577-60139 · 563 bp

Back to Rochelia

Pi 0.00888

MarkerSeek score 39.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 15
pi: 0.00888
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGGCAGA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.5	57.6	623-625 bp	1.0	66.9
petA-psbJ_p2	TGAGAAGGTTCAATTGGCAG	ATTCCGGATTGGGTTCATCC	45.0	50.0	55.9	57.6	623-625 bp	1.0	62.5
petA-psbJ_p3	TGAGAAGGTTCAATTGGCAGAA	ATTCCGGATTGGGTTCATCC	40.9	50.0	58.2	57.6	623-625 bp	1.0	61.7
petA-psbJ_p4	TTGAGAAGGTTCAATTGGCAGA	ATTCCGGATTGGGTTCATCC	40.9	50.0	58.2	57.6	624-626 bp	1.0	61.7
petA-psbJ_p5	TTGAGAAGGTTCAATTGGCAG	ATTCCGGATTGGGTTCATCC	42.9	50.0	56.7	57.6	624-626 bp	1.0	60.7