Hotspot

trnL-UAA-trnF-GAA

LSC · 46410-46728 · 319 bp

Back to Rochelia

Pi 0.00502

MarkerSeek score 55.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 319
variable_sites: 3
pi: 0.00502
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TCTACCAGCTGAGCTATCCC	45.0	55.0	57.8	58.0	418-435 bp	1.0	82.7
trnL-UAA-trnF-GAA_p2	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	454-471 bp	1.0	82.0
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	45.0	50.0	57.8	58.1	469-486 bp	1.0	81.6
trnL-UAA-trnF-GAA_p4	ACATGTCAATACCGGCAACA	TCAGTCCTCTGCTCTACCAG	45.0	55.0	57.8	57.9	430-447 bp	1.0	81.6
trnL-UAA-trnF-GAA_p5	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	45.0	50.0	57.8	58.2	472-489 bp	1.0	81.0