Hotspot

ycf3-trnS-GGA

LSC · 42940-43834 · 895 bp

Back to Rochelia

Pi 0.00439

MarkerSeek score 40.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 895
variable_sites: 11
pi: 0.00439
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATCAAAGGTGTTCGGCCTA	AACCATTTGACCCTTCACCC	50.0	50.0	57.9	58.0	1966-2011 bp	1.0	83.3
ycf3-trnS-GGA_p2	AGTCACTTCAACAGCCTTCG	AACCATTTGACCCTTCACCC	50.0	50.0	58.1	58.0	1886-1931 bp	1.0	83.3
ycf3-trnS-GGA_p3	TTCGGTGGTTCTACGGGTAT	AACCATTTGACCCTTCACCC	50.0	50.0	58.1	58.0	1870-1915 bp	1.0	83.2
ycf3-trnS-GGA_p4	CATCAAAGGTGTTCGGCCTA	TTCGATTGGGTATGGCTTCG	50.0	50.0	57.9	58.1	2163-2208 bp	0.833333	74.4
ycf3-trnS-GGA_p5	CCCGAGCCCAATAAGGAAAA	AACCATTTGACCCTTCACCC	50.0	50.0	57.9	58.0	1825-1841 bp	0.5	61.7