Hotspot

atpF-atpH

LSC · 12396-12784 · 389 bp

Back to Rochelia

Pi 0.00428

MarkerSeek score 30.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 5
pi: 0.00428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCCAAAGAAACGAAAGAATC	GGACTGGTTGTAGCATTAGCA	42.9	47.6	56.1	58.0	473-479 bp	1.0	49.2
atpF-atpH_p2	GCCCAAAGAAACGAAAGAATC	TGGACTGGTTGTAGCATTAGC	42.9	47.6	56.1	58.0	474-480 bp	1.0	49.2
atpF-atpH_p3	GCCCAAAGAAACGAAAGAATC	CTGAGGGAAAAATACGAGGCA	42.9	47.6	56.1	58.0	538-544 bp	1.0	49.1
atpF-atpH_p4	GCCCAAAGAAACGAAAGAATC	TGAGGGAAAAATACGAGGCA	42.9	45.0	56.1	56.8	537-543 bp	1.0	47.9
atpF-atpH_p5	GCCCAAAGAAACGAAAGAATC	AGCTGAGGGAAAAATACGAGG	42.9	47.6	56.1	57.7	540-546 bp	1.0	47.0