Hotspot

rps16

LSC · 5126-6224 · 1099 bp

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Pi 0.00468

MarkerSeek score 48.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1099
variable_sites: 11
pi: 0.00468
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAAATCACTATCCTACCCTTAGT	GTCTATGTACCGAGCGAACC	37.5	55.0	56.1	57.9	1227-1230 bp	1.0	40.9
rps16_p2	AGAAATCACTATCCTACCCT	GTCTATGTACCGAGCGAACC	40.0	55.0	52.0	57.9	1227-1230 bp	1.0	40.9
rps16_p3	AGAAATCACTATCCTACCCTT	GTCTATGTACCGAGCGAACC	38.1	55.0	53.0	57.9	1227-1230 bp	1.0	40.9
rps16_p4	AGAAATCACTATCCTACCCTTAGT	CCGAGCGAACCAATGACTAT	37.5	50.0	56.1	57.8	1218-1221 bp	1.0	40.9
rps16_p5	AGAAATCACTATCCTACCCTTAGT	TCTATGTACCGAGCGAACCA	37.5	50.0	56.1	58.2	1226-1229 bp	1.0	40.9