Hotspot

ccsA

SSC · 120999-121967 · 969 bp

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Pi 0.00289

MarkerSeek score 54.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 6
pi: 0.00289
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGTGATTCACGTGGTTCTCG	GCGGCATGCCATCTTCTAAA	50.0	50.0	57.9	59.0	1188-1191 bp	1.0	72.1
ccsA_p2	AGTGATTCACGTGGTTCTCG	GCGGCATGCCATCTTCTAA	50.0	52.6	57.9	58.3	1188-1191 bp	1.0	69.5
ccsA_p3	AGTGATTCACGTGGTTCTCG	CGGCATGCCATCTTCTAAAAG	50.0	47.6	57.9	57.7	1187-1190 bp	1.0	69.1
ccsA_p4	AGTGATTCACGTGGTTCTCG	CGGCATGCCATCTTCTAAAA	50.0	45.0	57.9	56.5	1187-1190 bp	1.0	67.7
ccsA_p5	TGATTCACGTGGTTCTCGC	GCGGCATGCCATCTTCTAAA	52.6	50.0	58.2	59.0	1186-1189 bp	1.0	63.2