Hotspot

atpF

LSC · 26922-28180 · 1259 bp

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Pi 0

MarkerSeek score 28.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1259
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGGCAAAGTAAAAAGAACTCTGT	TGCCTAAAGTACTAAAGTAGAAGGA	39.1	36.0	57.5	57.0	1341 bp	1.0	40.0
atpF_p2	AGGGCAAAGTAAAAAGAACTCTG	TGCCTAAAGTACTAAAGTAGAAGGA	39.1	36.0	57.2	57.0	1342 bp	1.0	40.0
atpF_p3	GGGCAAAGTAAAAAGAACTCTGT	TGCCTAAAGTACTAAAGTAGAAGG	39.1	37.5	57.5	55.6	1341 bp	1.0	40.0
atpF_p4	GGGCAAAGTAAAAAGAACTCTGT	GCCTAAAGTACTAAAGTAGAAGGA	39.1	37.5	57.5	55.4	1340 bp	1.0	40.0
atpF_p5	GGGCAAAGTAAAAAGAACTCTGT	ATGCCTAAAGTACTAAAGTAGAAGGA	39.1	34.6	57.5	57.4	1342 bp	1.0	40.0