Hotspot

rps18-rpl20

LSC · 67412-67705 · 294 bp

Back to Robinsonia

Pi 0.0152

MarkerSeek score 60.70

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 294
variable_sites: 9
pi: 0.0152
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AACCGAGTCGACCACTAGAA	TCTGTACAAGAGACAGTTGCT	50.0	42.9	58.1	56.6	435-459 bp	1.0	65.4
rps18-rpl20_p2	AAGAACCGAGTCGACCACTA	TCTGTACAAGAGACAGTTGCT	50.0	42.9	58.1	56.6	438-462 bp	1.0	65.3
rps18-rpl20_p3	TTGAAAGAACCGAGTCGACC	TCTGTACAAGAGACAGTTGCT	50.0	42.9	57.9	56.6	442-466 bp	1.0	64.9
rps18-rpl20_p4	AACCGAGTCGACCACTAGAA	TCTGTACAAGAGACAGTTGCTT	50.0	40.9	58.1	57.3	435-459 bp	1.0	63.1
rps18-rpl20_p5	AACCGAGTCGACCACTAGAA	TGTACAAGAGACAGTTGCTTCT	50.0	40.9	58.1	57.3	433-457 bp	1.0	63.1