Hotspot

psbE-petL

LSC · 63811-65040 · 1230 bp

Back to Robinsonia

Pi 0.00298

MarkerSeek score 50.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 8
pi: 0.00298
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.9	56.6	1376-1395 bp	1.0	69.0
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1299-1318 bp	1.0	68.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCCGTTAGTAGAAAACCGA	45.0	45.0	58.2	56.6	1385-1404 bp	1.0	68.0
psbE-petL_p4	TGCTGACGAATAACCAACCC	AGCCGTTAGTAGAAAACCGA	50.0	45.0	58.2	56.6	1390-1409 bp	1.0	67.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1384-1403 bp	1.0	67.9