Hotspot

trnR-UCU-trnS-CGA

LSC · 29987-30189 · 203 bp

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Pi 0.0102
MarkerSeek score 47.20
Primer available yes
Label trnR-UCU-trnS-CGA

Feature Metadata

label_name
trnR-UCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
203
variable_sites
5
pi
0.0102
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnS-CGA_p1 AGAAGACCTCTGTCCTATCCA AGCCTTCCAAGCTAACGATG 47.6 50.0 56.9 58.0 284-285 bp 1.0 64.8
trnR-UCU-trnS-CGA_p2 AGAAGACCTCTGTCCTATCCA CCTAGCCTTCCAAGCTAACG 47.6 55.0 56.9 58.1 287-288 bp 1.0 64.5
trnR-UCU-trnS-CGA_p3 AGGTTTAGAAGACCTCTGTCCT AGCCTTCCAAGCTAACGATG 45.5 50.0 57.9 58.0 290-291 bp 1.0 64.1
trnR-UCU-trnS-CGA_p4 AGGTTTAGAAGACCTCTGTCCT CCTAGCCTTCCAAGCTAACG 45.5 55.0 57.9 58.1 293-294 bp 1.0 63.8
trnR-UCU-trnS-CGA_p5 AGAAGACCTCTGTCCTATCCA CCCTAGCCTTCCAAGCTAAC 47.6 55.0 56.9 57.7 288-289 bp 1.0 62.3