Hotspot

atpA-trnR-UCU

LSC · 29791-29914 · 124 bp

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Pi 0.029

MarkerSeek score 66.40

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 7
pi: 0.029
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TACTTCAGGAACAAGCAGCC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	162-170 bp	1.0	72.3
atpA-trnR-UCU_p2	CATTCACCGAGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.9	57.3	225-233 bp	1.0	72.1
atpA-trnR-UCU_p3	AAGACATTCACCGAGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.9	57.3	229-237 bp	1.0	72.0
atpA-trnR-UCU_p4	CAGGAACAGAGGGAACGTTT	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.7	57.3	184-192 bp	1.0	71.4
atpA-trnR-UCU_p5	ATTCACCGAGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.6	57.3	224-232 bp	1.0	69.8