Hotspot

ycf3

LSC · 52508-54474 · 1967 bp

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Pi 0.0122

MarkerSeek score 52.30

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1967
variable_sites: 24
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GAAGTCAATCCGAGGCAAGT	TCGGCCATCTCTCCTTCATA	50.0	50.0	57.9	57.6	2943-2989 bp	1.0	77.5
ycf3_p2	GAAGTCAATCCGAGGCAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.9	57.8	2967 bp	0.5	57.7
ycf3_p3	AAGTCAATCCGAGGCAAGTG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.2	57.8	2966 bp	0.5	57.0
ycf3_p4	AATCCGAGGCAAGTGTTCAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.2	57.8	2961 bp	0.5	57.0
ycf3_p5	TCAATCCGAGGCAAGTGTTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.2	57.8	2963 bp	0.5	56.9