Hotspot

ndhF

SSC · 110695-112941 · 2247 bp

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Pi 0.0116

MarkerSeek score 57.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 26
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCAGTTATTAAACCCCCTTTT	TGCACTTTTTATTAACCGAACCC	33.3	39.1	52.6	57.9	2399-2401 bp	1.0	40.9
ndhF_p2	TTCAGTTATTAAACCCCCTTT	TGCACTTTTTATTAACCGAACCC	33.3	39.1	52.6	57.9	2400-2402 bp	1.0	40.9
ndhF_p3	TTTCAGTTATTAAACCCCCTT	TGCACTTTTTATTAACCGAACCC	33.3	39.1	52.6	57.9	2401-2403 bp	1.0	40.9
ndhF_p4	TTTTCAGTTATTAAACCCCCT	TGCACTTTTTATTAACCGAACCC	33.3	39.1	52.6	57.9	2402-2404 bp	1.0	40.9
ndhF_p5	TCAGTTATTAAACCCCCTTTT	ACCGAACCCTTTACAAAAGA	33.3	40.0	52.6	54.8	2385-2387 bp	1.0	40.9