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psbT-psbN

LSC · 74900-74965 · 66 bp

Back to Robinia

Pi 0.0606

MarkerSeek score 68.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 4
pi: 0.0606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTCGAAGGGCAGAATCGAAG	TCCCCTACAACAGTTCGTCT	50.0	50.0	58.0	58.0	1010 bp	1.0	80.4
psbT-psbN_p2	TCGTTTGCTCTGCTGTTCTT	TCCCCTACAACAGTTCGTCT	45.0	50.0	58.0	58.0	789 bp	1.0	80.3
psbT-psbN_p3	TTCGTTTGCTCTGCTGTTCT	TCCCCTACAACAGTTCGTCT	45.0	50.0	58.0	58.0	790 bp	1.0	80.3
psbT-psbN_p4	TATGGTGGCGAACTCAATGG	TCCCCTACAACAGTTCGTCT	50.0	50.0	58.0	58.0	954 bp	1.0	80.2
psbT-psbN_p5	TTCGAAGGGCAGAATCGAAG	ACAACAGTTCGTCTTGGACC	50.0	50.0	58.0	58.1	1004 bp	1.0	80.1