Hotspot

trnP-UGG-psaJ

LSC · 67087-67544 · 458 bp

Back to Robinia

Pi 0.0111

MarkerSeek score 56.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 5
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	581-621 bp	1.0	80.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	580-620 bp	1.0	80.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	592-632 bp	1.0	80.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	591-631 bp	1.0	80.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GCTAAAGCCCCGAACCATAA	50.0	50.0	58.1	58.0	594-634 bp	1.0	80.6