Hotspot

psbE-petL

LSC · 64884-66253 · 1370 bp

Back to Robinia

Pi 0.0156

MarkerSeek score 56.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1370
variable_sites: 21
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCGAAATAACTAGTTATAGTGGGC	50.0	41.7	57.8	57.0	1413-1423 bp	1.0	42.4
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CCGAAATAACTAGTTATAGTGGGC	55.0	41.7	58.2	57.0	1408-1418 bp	1.0	42.4
psbE-petL_p3	TGACAAATAACCAACCCGCA	CCGAAATAACTAGTTATAGTGGGC	45.0	41.7	58.0	57.0	1501-1511 bp	1.0	42.2
psbE-petL_p4	CAACCCGCAATGAATAGGGA	CCGAAATAACTAGTTATAGTGGGC	50.0	41.7	57.9	57.0	1490-1500 bp	1.0	42.2
psbE-petL_p5	ACCAACCCGCAATGAATAGG	CCGAAATAACTAGTTATAGTGGGC	50.0	41.7	58.2	57.0	1492-1502 bp	1.0	42.2