Hotspot

accD-psaI

LSC · 59313-59764 · 452 bp

Back to Robinia

Pi 0.0244

MarkerSeek score 65.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 11
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	574-928 bp	1.0	75.1
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	580-934 bp	1.0	74.5
accD-psaI_p3	CCACGCTTTCTTTCCTTTGAC	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.0	58.0	571-925 bp	1.0	73.2
accD-psaI_p4	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	574-928 bp	1.0	72.6
accD-psaI_p5	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	572-926 bp	1.0	72.6