Hotspot

trnQ-UUG-accD

LSC · 56466-57809 · 1344 bp

Back to Robinia

Pi 0.024
MarkerSeek score 64.30
Primer available yes
Label trnQ-UUG-accD

Feature Metadata

label_name
trnQ-UUG-accD
feature_type
igs
region
LSC
length_bp
1344
variable_sites
32
pi
0.024
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-accD_p1 GAGGTTCGAATCCTTCCGTC ATTGAACCGCCATTTTTCCA 55.0 40.0 58.1 56.5 1389-1403 bp 1.0 69.9
trnQ-UUG-accD_p2 GAGGTTCGAATCCTTCCGTC TTGAACCGCCATTTTTCCAT 55.0 40.0 58.1 56.5 1388-1402 bp 1.0 69.9
trnQ-UUG-accD_p3 GAGGTTCGAATCCTTCCGTC AATTGAACCGCCATTTTTCCA 55.0 38.1 58.1 57.2 1390-1404 bp 1.0 67.9
trnQ-UUG-accD_p4 GAGGTTCGAATCCTTCCGTC ATTGAACCGCCATTTTTCCAT 55.0 38.1 58.1 57.0 1389-1403 bp 1.0 66.1
trnQ-UUG-accD_p5 GAGGTTCGAATCCTTCCGTC AGAATTGAACCGCCATTTTTCC 55.0 40.9 58.1 58.1 1392-1406 bp 1.0 65.4