Hotspot

trnT-GGU-psbD

LSC · 40828-42270 · 1443 bp

Back to Robinia

Pi 0.0147

MarkerSeek score 64.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1443
variable_sites: 21
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ATAGACCAGACCAACCCACA	55.0	50.0	58.1	58.0	1610-1638 bp	1.0	80.7
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ATAGACCAGACCAACCCACA	50.0	50.0	58.1	58.0	1613-1641 bp	1.0	80.5
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CCAACCCACAAAAACGAACC	55.0	50.0	58.1	58.1	1600-1628 bp	1.0	80.5
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CCAACCCACAAAAACGAACC	50.0	50.0	58.1	58.1	1603-1631 bp	1.0	80.3
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AGACCAACCCACAAAAACGA	55.0	45.0	58.1	57.9	1603-1631 bp	1.0	80.0