Hotspot

trnF-GAA-trnL-UAA

LSC · 13118-13507 · 390 bp

Back to Robinia

Pi 0.0359

MarkerSeek score 71.70

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 14
pi: 0.0359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TCTACCAGCTGAGCTATCCC	AATCGTGAGGGTTCAAGTCC	55.0	50.0	58.0	57.5	444 bp	1.0	78.7
trnF-GAA-trnL-UAA_p2	TTTGAACTGGTGACACGAGG	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	480 bp	1.0	78.1
trnF-GAA-trnL-UAA_p3	GTGCCAGGAACCAGATTTGA	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	495 bp	1.0	77.7
trnF-GAA-trnL-UAA_p4	TCAGTCCTCTGCTCTACCAG	AATCGTGAGGGTTCAAGTCC	55.0	50.0	57.9	57.5	456 bp	1.0	77.6
trnF-GAA-trnL-UAA_p5	CATGTGCCAGGAACCAGATT	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.2	57.5	498 bp	1.0	77.2