Hotspot

trnV-UAC-ndhC

LSC · 10190-10602 · 413 bp

Back to Robinia

Pi 0.041

MarkerSeek score 76.20

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 413
variable_sites: 15
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	CCTTTGCTAATCGGGGCTAA	50.0	50.0	57.8	57.7	536-556 bp	1.0	81.9
trnV-UAC-ndhC_p2	GAAGGTCTACGGTTCGAGTC	CCTTTGCTAATCGGGGCTAA	55.0	50.0	57.5	57.7	535-555 bp	1.0	79.1
trnV-UAC-ndhC_p3	AGAAGGTCTACGGTTCGAGT	CTCTGGCCCTTTGCTAATCG	50.0	55.0	57.8	58.7	543-563 bp	1.0	79.0
trnV-UAC-ndhC_p4	AGAAGGTCTACGGTTCGAGT	TCTCTGGCCCTTTGCTAATC	50.0	50.0	57.8	57.3	544-564 bp	1.0	78.8
trnV-UAC-ndhC_p5	CGGTTCGAGTCCGTATAGTC	CCTTTGCTAATCGGGGCTAA	55.0	50.0	57.3	57.7	526-546 bp	1.0	78.2