Hotspot

trnK-UUU-rbcL

LSC · 4427-5028 · 602 bp

Back to Robinia

Pi 0.0257

MarkerSeek score 63.30

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 15
pi: 0.0257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGCCGAGTACTCTACCATTGA	GAATTCCCAGCAATGGATAC	47.6	45.0	58.0	54.1	661-1083 bp	1.0	43.7
trnK-UUU-rbcL_p2	AATGGAGTCGCGCTTAAAAG	GAATTCCCAGCAATGGATAC	45.0	45.0	56.8	54.1	679-1101 bp	1.0	43.0
trnK-UUU-rbcL_p3	GCCGAGTACTCTACCATTGAG	GAATTCCCAGCAATGGATAC	52.4	45.0	57.6	54.1	660-1082 bp	1.0	43.0
trnK-UUU-rbcL_p4	AAATGGAGTCGCGCTTAAAAG	GAATTCCCAGCAATGGATAC	42.9	45.0	57.5	54.1	680-1102 bp	1.0	43.0
trnK-UUU-rbcL_p5	GCCGAGTACTCTACCATTGA	GAATTCCCAGCAATGGATAC	50.0	45.0	56.5	54.1	660-1082 bp	1.0	43.0