Hotspot

rbcL

LSC · 58173-59615 · 1443 bp

Back to Ripogonum

Pi 0.0037

MarkerSeek score 49.10

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1443
variable_sites: 14
pi: 0.0037
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1529 bp	1.0	72.0
rbcL_p2	TGAGTTGTAGGGAGGGACTT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.3	58.4	1530 bp	1.0	72.0
rbcL_p3	TGAGTTGTAGGGAGGGACTT	GGAAAAGATTGGGCCGAGTT	50.0	50.0	57.3	58.5	1538 bp	1.0	71.4
rbcL_p4	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1532 bp	1.0	70.2
rbcL_p5	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.1	58.4	1533 bp	1.0	70.2