Hotspot

psbC

LSC · 35765-37186 · 1422 bp

Back to Ripogonum

Pi 0.000469

MarkerSeek score 33.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.000469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTTCCCAAGAAATCCGTGCA	AGCCGAGCCAGAACATAAAA	45.0	45.0	58.0	57.5	1595 bp	1.0	76.1
psbC_p2	AAGAAATCCGTGCAGCAGAA	AGCCGAGCCAGAACATAAAA	45.0	45.0	58.1	57.5	1589 bp	1.0	75.4
psbC_p3	GTGCAGCAGAAGATCCTGAA	AGCCGAGCCAGAACATAAAA	50.0	45.0	57.9	57.5	1580 bp	1.0	75.3
psbC_p4	AAATCCGTGCAGCAGAAGAT	AGCCGAGCCAGAACATAAAA	45.0	45.0	57.9	57.5	1586 bp	1.0	75.2
psbC_p5	AGGTATTCGTGCTTGGATGG	AGCCGAGCCAGAACATAAAA	50.0	45.0	57.7	57.5	1522 bp	1.0	73.5