Hotspot

ndhK

LSC · 51792-52493 · 702 bp

Back to Ripogonum

Pi 0.00171

MarkerSeek score 30.40

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 702
variable_sites: 3
pi: 0.00171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	ATGAAATCTGGAGTCGTGCC	GACCGGAGAAGCTCTCTAGT	50.0	55.0	58.0	58.0	1624 bp	1.0	80.1
ndhK_p2	ATGAAATCTGGAGTCGTGCC	CACCGATTAGTGAAGGACCG	50.0	55.0	58.0	58.1	1639 bp	1.0	79.8
ndhK_p3	CCTTGGTAATCGAAGCCCAA	GACCGGAGAAGCTCTCTAGT	50.0	55.0	57.9	58.0	1168 bp	1.0	79.4
ndhK_p4	AATGACAGCAATGGAGTCCC	GACCGGAGAAGCTCTCTAGT	50.0	55.0	57.9	58.0	1220 bp	1.0	79.3
ndhK_p5	GCCAGCCTATCCAACTTTCA	GACCGGAGAAGCTCTCTAGT	50.0	55.0	57.9	58.0	1515 bp	1.0	79.2