Hotspot

ndhF

SSC · 115579-117816 · 2238 bp

Back to Ripogonum

Pi 0.00223

MarkerSeek score 52.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 11
pi: 0.00223
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGTTCTATTCACGTGCAAACA	AGAATTGGTGAATCGGAAACA	38.1	38.1	56.1	55.8	2392-2393 bp	1.0	40.4
ndhF_p2	AGTTCTATTCACGTGCAAACA	AAGAATTGGTGAATCGGAAACA	38.1	36.4	56.1	56.5	2393-2394 bp	1.0	40.4
ndhF_p3	AGTTCTATTCACGTGCAAACA	AGAATTGGTGAATCGGAAACAA	38.1	36.4	56.1	56.5	2392-2393 bp	1.0	40.4
ndhF_p4	AAGTTCTATTCACGTGCAAACA	AGAATTGGTGAATCGGAAACA	36.4	38.1	56.8	55.8	2393-2394 bp	1.0	40.4
ndhF_p5	AGTTCTATTCACGTGCAAACAT	AGAATTGGTGAATCGGAAACA	36.4	38.1	56.6	55.8	2392-2393 bp	1.0	40.4